Limits to Sulfur Accumulation in Transgenic Lupin Seeds ...
Northernblot analysis was performed on total RNA extracted from developing cotyledons by grinding plant tissue to a fine power in liquid nitrogen using a mortar and pestle then extracting into the following buffer: 1 m TrisHCl, pH ; 1% (w/v) SDS; and % (v/v) βmercaptoethanol. Protein was removed by extraction with 2 volumes of a ...