Immunoprecipitation Protocol Utilizing Magnetic Separation ...
IMPORTANT: Prewash #73778 and #70024 magnetic beads just prior to use: Transfer 20 μl of bead slurry to a clean tube. Place the tube in a magnetic separation rack for 1015 seconds. Carefully remove the buffer once the solution is clear. Add 500 μl of 1X cell lysis buffer to the magnetic bead pellet, briefly vortex to wash the beads.